Anti-KIF1C 抗体 (ab72238)
Key features and details
- Rabbit polyclonal to KIF1C
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF1C antibody
KIF1C 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF1C -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human KIF1C aa 850-950.
Database link: O43896 -
ポジティブ・コントロール
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72238の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Motor required for the retrograde transport of Golgi vesicles to the endoplasmic reticulum. Has a microtubule plus end-directed motility. -
組織特異性
Expressed in all tissues examined, with most abundant expression in heart and skeletal muscle. -
関連疾患
Spastic ataxia 2, autosomal recessive -
配列類似性
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Unc-104 subfamily.
Contains 1 FHA domain.
Contains 1 kinesin motor domain. -
翻訳後修飾
Phosphorylated on tyrosine residues. -
細胞内局在
Cytoplasm, cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 10749 Human
- Omim: 603060 Human
- SwissProt: O43896 Human
- Unigene: 435120 Human
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別名
- Kif1c antibody
- KIF1C_HUMAN antibody
- Kinesin family member 1C antibody
see all
画像
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All lanes : Anti-KIF1C antibody (ab72238) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 123 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Various other bands are detected from 31 kDa upwards. -
Immunoprecipitation/ Western Blot of KIF1C.
Lane 1: ab72238 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72238 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab72238 は 2 報の論文で使用されています。
- Gifford V et al. Coordination of two kinesin superfamily motor proteins, KIF3A and KIF13A, is essential for pericellular matrix degradation by membrane-type 1 matrix metalloproteinase (MT1-MMP) in cancer cells. Matrix Biol 107:1-23 (2022). PubMed: 35122963
- Jones LA et al. Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion. J Cell Biol 207:499-516 (2014). PubMed: 25422374