Anti-KIF1A 抗体 (ab91029)
Key features and details
- Rabbit polyclonal to KIF1A
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF1A antibody
KIF1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF1A -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, WB, ELISAmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog -
免疫原
Synthetic peptide corresponding to Human KIF1A aa 1500-1600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab108241) -
ポジティブ・コントロール
- This antibody gave a positive signal in both Mouse brain embryonic E14 and Mouse brain embryonic E18 tissue lysates. As well as human hippocampus formalin fixed paraffin embedded tissue section within immunohistochemistry.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91029の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).
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ELISA |
Use a concentration of 1 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa). |
ELISA
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Motor for anterograde axonal transport of synaptic vesicle precursors. -
配列類似性
Belongs to the kinesin-like protein family. Unc-104 subfamily.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Contains 1 PH domain. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 547 Human
- Entrez Gene: 16560 Mouse
- Omim: 601255 Human
- SwissProt: Q12756 Human
- SwissProt: P33173 Mouse
- Unigene: 516802 Human
- Unigene: 276408 Mouse
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別名
- ATSV antibody
- Axonal transporter of synaptic vesicles antibody
- C2orf20 antibody
see all
画像
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All lanes : Anti-KIF1A antibody (ab91029) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 191 kDa
Observed band size: 191 kDa
Additional bands at: 58 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
IHC image of KIF1A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91029, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ab91029 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 1% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab91029 は 2 報の論文で使用されています。
- Lannom MC et al. FMRP and MOV10 regulate Dicer1 expression and dendrite development. PLoS One 16:e0260005 (2021). PubMed: 34847178
- Hung CO & Coleman MP KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons. Traffic 17:1155-1167 (2016). WB ; Mouse . PubMed: 27484852