Anti-KCNN2/SK2 抗体 (ab99457)
Key features and details
- Goat polyclonal to KCNN2/SK2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KCNN2/SK2 antibody
KCNN2/SK2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to KCNN2/SK2 -
由来種
Goat -
特異性
Recognizes both Human isoforms (NP_067627.2 and NP_740721.1) -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Guinea pig, Cow, Dog, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human liver lysate. IHC-P: Human thyroid tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99457の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 404177 Cow
- Entrez Gene: 474640 Dog
- Entrez Gene: 3781 Human
- Entrez Gene: 140492 Mouse
- Entrez Gene: 100172000 Orangutan
- Entrez Gene: 54262 Rat
- Omim: 605879 Human
- SwissProt: Q6PJI0 Human
see all -
別名
- Apamin sensitive small conductance Ca2+ activated potassium antibody
- Apamin sensitive small conductance Ca2+ activated potassium channel antibody
- hSK2 antibody
see all
画像
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Anti-KCNN2/SK2 antibody (ab99457) at 0.3 µg/ml + Human liver lysate, in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 64, 26 kDa
Observed band size: 23,70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab99457 は 1 報の論文で使用されています。
- Du H et al. The Cation Channel TMEM63B Is an Osmosensor Required for Hearing. Cell Rep 31:107596 (2020). PubMed: 32375046