Application
Western blot
Loading amount
10 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Sample
Zebrafish Tissue lysate - whole (whole embryo lysate, deyolked, 10 embryos)
Specification
whole embryo lysate, deyolked, 10 embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-Tween 5% nonfat Milk
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: JAckson ImmunoResearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Bands
Specific: 300 kDa Non-specific: 80 kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
other stages do not show the specific band (not shown)
Positive control
/
Additional data
Additional Notes
1 - Shield stage, 10 embryos, antibody 1:500
2 - Shield stage, 10 embryos, antibody 1:1000
3 - Shield stage, 10 embryos, antibody 1:3000
Specific region at 300kDa shows two distinct bands
Abcam response
We appreciate the valuable feedback provided for the performance of ab61217 with zebrafish samples. We are pleased to see that the antibody worked well for this researcher. As the homology of the human and zebrafish KAT3B / p300 is only 45%-50 % we would welcome feedback from other researchers using this antibody with zebrafish samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Maximilian Krause
Verified customer
投稿 May 01 2014