Application
Western blot
Loading amount
10 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Sample
Zebrafish Tissue lysate - whole (whole embryo lysate, deyolked, 10 embryos)
Specification
whole embryo lysate, deyolked, 10 embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-Tween 5% nonfat Milk
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: JAckson ImmunoResearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Negative control
/
Positive control
p300 antibody ab61217 worked on the same samples (not shown)
Additional data
Additional Notes
1 - Shield lysate, 10 embryos, Dilution 1:500
2 - Shield lysate, 10 embryos, Dilution 1:1000
3 - Shield lysate, 10 embryos, Dilution 1:3000
Might work in ChIP
Abcam response
We appreciate the valuable feedback about the use of ab14984 with zebrafish samples provided in this Abreview. Regrettably the antibody did not give good results for this samples type. The data indicates that ab14984 might not be suitable for zebrafish samples. We would welcome additional feedback from other researchers working with zebrafish.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Maximilian Krause
Verified customer
投稿 Apr 28 2014