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All lanes : Anti-KAT13D / CLOCK antibody (ab93804) at 0.1 µg/ml
Lane 1 : HeLa at 50 µg
Lane 2 : HeLa at 15 µg
Lane 3 : HeLa at 5 µg
Lane 4 : 293T at 50 µg
developed using the ECL technique
Predicted band size : 95 kDa
Exposure time : 3 minutes
ab93804 at 1 µg/ml detecting KAT13D/CLOCK in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of KAT13D/CLOCK
Lane 2: ab93804 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
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