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Products:Neuroscience >> Neurology process >> Circadian Rhythm
Anti-KAT13D / CLOCK antibody - ChIP Grade (ab3517)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAT13D / CLOCK antibody (ab3517)
ab3517 staining KAT13D/CLOCK in Mouse skeletal muscle tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded tissue sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20ºC; antigen retrieval was by heat mediation in citrate buffer pH6. Samples were incubated with primary antibody (1/400 in PBS) for 12 hours at 4ºC. Undiluted ab64256 was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - KAT13D / CLOCK antibody (ab3517)
All lanes : Anti-KAT13D / CLOCK antibody - ChIP Grade (ab3517) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 95 kDa
Additional bands at : 105 kDa (possible post-translational modification),38 kDa,45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
KAT13D / CLOCK contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern observed at a higher molecular weight than predicted.
KAT13D / CLOCK antibody - ChIP Grade for Western blot in Mouse (3517)
KAT13D / CLOCK antibody for IHC-P in Mouse (3517)
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