Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skeletal muscle tissue)
Specification
Skeletal muscle tissue
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/400
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS
Secondary antibody
Name
Abcam antibody:Biotinylated Goat Anti Rabbit IgG (H+L) (Ready to use)
Additional data
Additional Notes
Secondary Ab was Biotinylated Goat Anti Rabbit IgG (ab64256)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Verified customer
投稿 Mar 18 2010