Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary rat hepatocyte)
Gel Running Conditions
Reduced Denaturing (9%)
Loading amount
20 µg
Treatment
1mM paracetamol
Specification
Primary rat hepatocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% BSA in TBS-T
Dilution
1/5000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Pico Chemiluminescent S
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 48, 40 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Philip Probert
Verified customer
投稿 Jun 03 2016