Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Treatment
Untreated Jurkat cells and cells treated with 10-8, 10-7, and 10-6 uM Methotrexate for 48 hours
Specification
Jurkat cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1.0 gram dried milk dissolved in PBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rb IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~55 and ~49 kDa
Positive control
B-actin.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 15 2011