Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary neurons)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Treatment
0.5M sorbitol
Specification
primary neurons
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
LiCor
Negative control
untreated
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: approx 55 and 45 kDa
Positive control
+ sorbitol
Additional data
Additional Notes
lane 1 - untreated control
lane 2 - sorbitol
Abcam response
Recommendation: Phospate groups in PBS can interfere with anti-phosphate antibody. To reduce background TBS could be tried.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 14 2010