Application
Western blot
Sample
Mouse Tissue lysate - whole (retina)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 54 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 10 2015