The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
1/500 - 1/1000.
1/50 - 1/100.
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins.
Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland.
Belongs to the JIP scaffold family. Contains 1 PID domain. Contains 1 SH3 domain.
Cytoplasm. Accumulates in cell surface projections.
C jun amino terminal kinase interacting protein 2 antibody
C-jun-amino-terminal kinase-interacting protein 2 antibody
Homologous to mouse JIP 1 antibody
IB 2 antibody
Islet brain 2 antibody
JIP 2 antibody
JNK interacting protein 2 antibody
JNK MAP kinase scaffold protein 2 antibody
JNK MAP kinase scaffold protein JIP2 antibody
JNK-interacting protein 2 antibody
Mitogen activated protein kinase 8 interacting protein 2 antibody
Mitogen-activated protein kinase 8-interacting protein 2 antibody
PRKM8 interacting protein like antibody
Western blot - JIP2 antibody (ab65211)
All lanes : Anti-JIP2 antibody (ab65211) at 1/500 dilution
Lane 1 : Extracts from mouse brain cells Lane 2 : Extracts from mouse brain cells, plus immunising peptide
Predicted band size: 88 kDa Observed band size: 88 kDa Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
Immunohistochemistry (Frozen sections) - JIP2 antibody (ab65211)This image is courtesy of an anonymous Abreview
ab65211 staining the JIP2 (Red) in Zebra finch basal ganglia (striatum) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.3% Triton and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS Tween) for 24 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (Blue).
ICC/IF image of ab65211 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65211, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.