Application
Western blot
Sample
Mouse Cell lysate - whole cell (CD4+ T cells)
Loading amount
1e+006 cells
Specification
CD4+ T cells
Gel Running Conditions
Non-reduced Denaturing (4 - 12 % gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: zymed anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: - kDa Non-specific: 100 kDa
Positive control
beta-actin
Additional data
Abcam response
Thank you for your valuable feedback. This antibody has not been tested for cross-reaction with mouse so we do not have any data on this species. Since you had good signal with positive control but this antibody failed to work in nuclear extracts from murine T cells, it is very likely that ab40916 does not recognize mouse JDP2.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 26 2008