Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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ab21996 |
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ab21996 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
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I have initially used this antibody from a colleague in my IHC-P experiment and it worked great. this was lot GR26251. Then I ordered my own vial, GR43585, but this lot did not work on the same sections. I see faint specific staining in the nucleus but high background in the nucleus as well. |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab20670. |
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Hi, I have two parts I am working on setting up in my system and have a question on the storage conditions. The info I received on both cat# has store at +4C short term but our storage condition range is 2-8C. Is it okay to store short term at our range 2-8? Please advise so I can move forward. CAT# AB20670 And CAT# AB92606 |
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ANSWER: |
Thank you for contacting Abcam. |
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It is for the cat# above in Subject Line. |
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ANSWER: |
Thanks for the additional information. I have attached a Certificate of Compliance for ab20670.
Please let me know if you need any further information. |
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Would it be possible to get a COC from any lot? |
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ANSWER: |
Thanks for your enquiry. Sure, we can provide a CoC from any lot. Please just include the product number and lot number you are interested in. |
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Can I get a copy of a COA from any lot for my set up? |
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ANSWER: |
We do have CoCs (Certificates of Compliance) for any lot you need but we do not have CoAs available. I hope this is helpful. Please contact me again if you have any further questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Dorsal root ganglion explants were dissected from 16 day-old rat embryos and cultured for 6 hours in vitro with Neurobasal Medium containing B27 supplement.
Nuclei stained positive for anti-Islet 1 antibody ab20670 at 2µg/ml. As would be expected, not all cells in this preparation were Islet 1-positive. Pre-incubation of ab20670 with the immunizing peptide ab21996 resulted in complete blocking of the antibody.
Green = ab20670
Blue = To-pro-3 nuclear stain
The level of magnification is different in each image.
Randal Moldrich, CNRS UMR7637, ESPCI, France
ab20670 detected Islet 1 in the nucleus of frozen sections of E11.5 mouse embryonic brain using 1 ug/ml of antibody. Brighter staining may be achieved using a greater concentration of antibody, e.g. 2.5 or 5 ug/ml.
ab20670 2µg/ml staining ISLET1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and weak cytoplasmic staining primarily in the pancreatic islet.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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