Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Permeabilization
Yes - saponin
Specification
Human pluripotent stem cell derived cardiomyocyte
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: commercial perm/wash buffer
Dilution
1/1200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor® 568 Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Distinct staining of ISL1 (red) in the nuclei (blue) of cardiomyocytes (NKX2-5-GFP) derived from human pluripotent stem cells. Nuclei negative for ISL1 staining can also be observed, indicating staining is not generic nuclear staining.
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投稿 Oct 16 2014