Anti-Ionotropic glutamate receptor 2 + 3 抗体 [EP929Y] (ab52896)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y]
Ionotropic glutamate receptor 2 + 3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3 -
由来種
Rabbit -
特異性
This antibody detects both GluR2 phosphorylated on Serine 880 + GluR3 phosphorylated on Serine 891 of the mature human, and also unphosphorylated GluR2 + 3.
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アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human brain and fetal tissue lysates and mouse and rat brain tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP929Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52896の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
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特記事項 |
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WB
1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). |
ターゲット情報
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関連性
Ion channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory. -
細胞内局在
Cell Membrane; multi-pass membrane protein. -
参照データベース
- Entrez Gene: 2891 Human
- Entrez Gene: 2892 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- Omim: 305915 Human
- SwissProt: P42262 Human
- SwissProt: P42263 Human
see all -
別名
- AMPA selective glutamate receptor 2 antibody
- AMPA selective glutamate receptor 3 antibody
- GluR K2 antibody
see all
画像
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All lanes : Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/5000 dilution (unpurified) + Human brain tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab52896 は 7 報の論文で使用されています。
- Moidunny S et al. Glycogen synthase kinase-3 inhibition rescues sex-dependent contextual fear memory deficit in human immunodeficiency virus-1 transgenic mice. Br J Pharmacol 177:5658-5676 (2020). PubMed: 33080056
- Wei C et al. MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer. Aging (Albany NY) 11:6734-6761 (2019). PubMed: 31498117
- Yang T et al. Functions of CaBP1 and CaBP2 in the peripheral auditory system. Hear Res 364:48-58 (2018). IHC-P ; Mouse . PubMed: 29661613
- Zhou JY et al. Individual and combined effects of rhynchophylline and ketamine on proliferation, NMDAR1 and GluA2/3 protein expression in PC12 cells. Fitoterapia 85:125-9 (2013). ICC/IF ; Rat . PubMed: 23352746
- Krosnowski K et al. Diverse populations of intrinsic cholinergic interneurons in the mouse olfactory bulb. Neuroscience 213:161-78 (2012). PubMed: 22525133
- Ogino K et al. Duplicated gephyrin genes showing distinct tissue distribution and alternative splicing patterns mediate molybdenum cofactor biosynthesis, glycine receptor clustering, and escape behavior in zebrafish. J Biol Chem 286:806-17 (2011). IHC-Fr ; Zebrafish . PubMed: 20843816
- Graham CE et al. A corticotropin-releasing factor system expressed in the cochlea modulates hearing sensitivity and protects against noise-induced hearing loss. Neurobiol Dis 38:246-58 (2010). IP ; Mouse . PubMed: 20109547