Application
IHC - Wholemount
Sample
Zebrafish Embryo (embryonic neural tube)
Specification
embryonic neural tube
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: :10% Serum in PBS with 0.1% TX-100 and
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
We tried whole mount IHC for 1day old zebrafish larvae with and without Permeabilization with 10´g/ml proteinase K for 20 min. There was high amount of background and almost no specific signal for ITSN1 in the neural tube tissue.
Image shows cells in the neural tube labelled with nuclear dye (white) and immunostaining for anti-ITSN1 (red) at 1 in 100 dilution. Scale bar - 20´m.
Abcam response
Thank you for participating in our AbTrial testing program. We will continue to gather data on use in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 03 2018