Anti-Integrin linked ILK 抗体 [EPR1592] (ab76468)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1592] to Integrin linked ILK
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Integrin linked ILK antibody [EPR1592]
Integrin linked ILK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1592] to Integrin linked ILK -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human Integrin linked ILK. The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899); HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1592 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76468の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/5000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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ICC/IF |
1/500.
For unpurified use at 1/100 - 1/250 |
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Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/5000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250 |
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. -
組織特異性
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
ドメイン
A PH-like domain is involved in phosphatidylinositol phosphate binding. -
翻訳後修飾
Autophosphorylated on serine residues. -
細胞内局在
Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
- Unigene: 5158 Human
see all -
別名
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
Lane 3: Hek293 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76468 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76468 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. ab76468 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labelling Integrin linked ILK (green) with purified ab76468 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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All lanes : Anti-Integrin linked ILK antibody [EPR1592] (ab76468) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
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ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
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Overlay histogram showing HEK293 cells stained with ab76468 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76468, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab76468 は 17 報の論文で使用されています。
- Zhang H et al. Silencing LncRNA CASC9 inhibits proliferation and invasion of colorectal cancer cells by MiR-542-3p/ILK. PLoS One 17:e0265901 (2022). PubMed: 35427373
- Huang LL et al. Integrin-Linked Kinase (ILK) Regulates Urinary Stem Cells Differentiation into Smooth Muscle via NF-?B Signal Pathway. Stem Cells Int 2021:6633111 (2021). PubMed: 33854551
- Ghazi A et al. Prognostic value of the PrPC-ILK-IDO1 axis in the mesenchymal colorectal cancer subtype. Oncoimmunology 10:1940674 (2021). PubMed: 34249475
- Liu S et al. Periostin regulates osteogenesis of mesenchymal stem cells from ovariectomized rats through actions on the ILK/Akt/GSK-3β Axis. Genet Mol Biol 44:e20200461 (2021). PubMed: 34591063
- Longaretti L et al. Unravelling the Role of PAX2 Mutation in Human Focal Segmental Glomerulosclerosis. Biomedicines 9:N/A (2021). PubMed: 34944624