Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (EpiSCs)
Specification
EpiSCs
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0.1%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa fluor 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 14 2011