Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hepatocellular carcinoma)
Permeabilization
Yes - Triton X-100
Specification
Hepatocellular carcinoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/15
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Alexa Fluor 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
We would like to thank the reviewer for submitting this AbReview. As the product has not yet been tested in this application, we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 22 2018