Application
Immunohistochemistry (Frozen sections)
Sample
Medaka fish Tissue sections (Basal side of epithelia)
Specification
Basal side of epithelia
Fixative
DMSO+Methanol
Permeabilization
Yes - acetone
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: invitrogen
Host species: Medaka fish
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Medaka fish
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
This antibody stained apical membrane rather than basal membrane of epithelia.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Makoto Furutani-Seiki
Verified customer
投稿 Nov 15 2011