Anti-Integrin alpha V + beta 6 抗体 [10D5] - BSA and Azide free (ab77906)
Key features and details
- Mouse monoclonal [10D5] to Integrin alpha V + beta 6 - BSA and Azide free
- Suitable for: Flow Cyt, IP, Blocking
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Integrin alpha V + beta 6 antibody [10D5] - BSA and Azide free
Integrin alpha V + beta 6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [10D5] to Integrin alpha V + beta 6 - BSA and Azide free -
由来種
Mouse -
特異性
ab77906 recognises alpha V beta 6 and does not recognize alpha V or other alpha V integrins. -
アプリケーション
適用あり: Flow Cyt, IP, Blockingmore details
適用なし: IHC-P or WB -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Tissue, cells or virus corresponding to Mouse Integrin alpha V + beta 6. Wild type murine keratinocytes.
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ポジティブ・コントロール
- H441 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Sterile filtered -
ポリ/モノ
モノクローナル -
クローン名
10D5 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77906の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture. |
|
Blocking |
Use at an assay dependent concentration.
Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP. |
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture. |
Blocking
Use at an assay dependent concentration. Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP. |
ターゲット情報
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機能
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3685 Human
- Entrez Gene: 3694 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 16420 Mouse
- Entrez Gene: 296456 Rat
- Entrez Gene: 311061 Rat
- Omim: 147558 Human
- Omim: 193210 Human
see all -
別名
- CD51 antibody
- Integrin alpha-V light chain antibody
- integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (16)
ab77906 は 16 報の論文で使用されています。
- Wang X et al. Inhibition of Integrin αvβ6 Activation of TGF-β Attenuates Tendinopathy. Adv Sci (Weinh) 9:e2104469 (2022). PubMed: 35166070
- Harvey Y et al. An Improved αvβ6-Receptor-Expressing Suspension Cell Line for Foot-and-Mouth Disease Vaccine Production. Viruses 14:N/A (2022). PubMed: 35337028
- Gringhuis SI et al. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs. Nat Immunol 23:1735-1748 (2022). PubMed: 36456734
- Bieri M et al. The RGD-binding integrins αvβ6 and αvβ8 are receptors for mouse adenovirus-1 and -3 infection. PLoS Pathog 17:e1010083 (2021). PubMed: 34910784
- Hägglund S et al. Model of persistent foot-and-mouth disease virus infection in multilayered cells derived from bovine dorsal soft palate. Transbound Emerg Dis 67:133-148 (2020). PubMed: 31419374