Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
doesn't work
Discount code: 53824
The second (positive control) staining was set up using Alexa Fluor® 488 goat anti-rabbit IgG.
Discount code: 53824
The second (positive control) staining was set up using Alexa Fluor® 488 goat anti-rabbit IgG.
Abcam response
Thank you for your valuable feedback and for participating in our Abreview testing discount program. We are sorry to hear that this antibody has not given you good results in immunocytochemistry using mouse sample. This antibody has not been tested with this species before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 24 2011