Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (developing pancreas E14/15)
Specification
developing pancreas E14/15
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: I0mM TRIS pH10
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/1250
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
In my hands, Citric acid HIER pH6 also worked but there was also non-specific immunostaining of occassional developing exocrine cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jun 26 2007