Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/1500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide pH7.5
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Ab8304 (1/1500) staining Insulin + Proinsulin antibody (D6C4) in Immunohistochemistry Paraffin-embedded Rat Pancreas tissue section.
Using a std stABCpx-DAB detection system there is clear positivity in Islets of Langerhans.
I detect no non-specific imunostaining in rat Pancreas.
Sections were pre-incubated in my endogenous biotin-blocking solutions.
See here http://www.immunoportal.com/index.php in IHC Protocols for my in-house procedure.
Using a std stABCpx-DAB detection system there is clear positivity in Islets of Langerhans.
I detect no non-specific imunostaining in rat Pancreas.
Sections were pre-incubated in my endogenous biotin-blocking solutions.
See here http://www.immunoportal.com/index.php in IHC Protocols for my in-house procedure.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Mar 11 2010