Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3 J2)
Permeabilization
Yes - 0,15% Triton X-100, 5 min
Specification
NIH 3T3 J2
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: Normal Goat Serum 10%
Dilution
1/250
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-Rabbit 550 Thermo SA5-100 33, Dylight 550
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 550
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 550
Additional data
Additional Notes
Fixative: 4% paraformaldehyde, 4% glucose.
Abcam response
Thank you for submitting your IF data in mouse. We have not tested this application and will continue to gather data on this use. To improve your staining I would suggest using 0.25% Triton for 10 minutes and an overnight incubation with the primary antibody. Thank you again for your feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Simon Boudreault
Verified customer
投稿 Dec 02 2015