Anti-Importin 13 抗体 (ab95993)
Key features and details
- Rabbit polyclonal to Importin 13
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Importin 13 antibody
Importin 13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Importin 13 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Synthetic peptide containing a sequence corresponding to a region within amino acids 901-963 (SMWIKEALQP PGFPSARLSP EQKDTFSQQI LRERVNKRRV KEMVKEFTLL CRGLHGTDYT ADY) of Human Importin 13 (NP_055467).
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035); HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95993の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 108 kDa.
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IP |
Use at 10-1 µg/mg of lysate.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 108 kDa. |
IP
Use at 10-1 µg/mg of lysate. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. -
組織特異性
Expressed in fetal brain, heart, intestine and kidney. -
配列類似性
Belongs to the importin beta family.
Contains 14 HEAT repeats.
Contains 1 importin N-terminal domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 523399 Cow
- Entrez Gene: 9670 Human
- Entrez Gene: 230673 Mouse
- Entrez Gene: 116458 Rat
- GenBank: NM_014652 Human
- Omim: 610411 Human
- SwissProt: A7YWD2 Cow
- SwissProt: O94829 Human
see all -
別名
- Imp 13 antibody
- Imp13 antibody
- Importin-13 antibody
see all
画像
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All lanes : Anti-Importin 13 antibody (ab95993)
Lane 1 : Non-transfected lysate
Lane 2 : IPO13 transfected lysate
Predicted band size: 108 kDa -
Importin 13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 µg of ab95993. The precipitated Importin 13 was detected with ab95993 at 1/1000 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab95993 at 1/200 dilution. Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab95993 は 2 報の論文で使用されています。
- Kalita J et al. On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions. J Cell Sci 134:N/A (2021). PubMed: 33912945
- Xue J et al. Forkhead Box M1 Is Essential for Nuclear Localization of Glioma-associated Oncogene Homolog 1 in Glioblastoma Multiforme Cells by Promoting Importin-7 Expression. J Biol Chem 290:18662-70 (2015). PubMed: 26085085