Anti-Iduronate 2 sulfatase 抗体 (ab85701)

製品の概要

  • 製品名
    Anti-Iduronate 2 sulfatase antibody
    Iduronate 2 sulfatase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Iduronate 2 sulfatase
  • 特異性
    Expected to recognize isoform A (NP_000193.1).
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    KHFRFRDLEEDP

    by a Cysteine residue linker, corresponding to internal sequence amino acids 440-451 of Human Iduronate 2 sulfatase (NP_000193.1)

  • ポジティブ・コントロール
    • Human Liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

ターゲット情報

  • 機能
    Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.
  • 組織特異性
    Liver, kidney, lung, and placenta.
  • 関連疾患
    Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2) [MIM:309900]; also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation.
  • 配列類似性
    Belongs to the sulfatase family.
  • 翻訳後修飾
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • 細胞内局在
    Lysosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha L iduronate sulfate sulfatase antibody
    • Alpha-L-iduronate sulfate sulfatase antibody
    • AW214631 antibody
    • Ids antibody
    • IDS_HUMAN antibody
    • Iduronate 2 sulfatase 14 kDa chain antibody
    • Iduronate 2 sulfatase 42 kDa chain antibody
    • Iduronate 2 sulfatase antibody
    • Iduronate 2-sulfatase 14 kDa chain antibody
    • Iduronate sulfatase antibody
    • Idursulfase antibody
    • MPS2 antibody
    • RP23-29M4.1 antibody
    • SIDS antibody
    see all

画像

  • Anti-Iduronate 2 sulfatase antibody (ab85701) at 0.1 µg/ml + Human Liver lysate at 35 µg

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab85701 (3.8µg/ml) staining of paraffin embedded Human Placenta shows lysosomal staining of trophoblasts. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

参考文献

ab85701 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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