Anti-Iba1 抗体 (ab108539)
Key features and details
- Rabbit polyclonal to Iba1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Iba1 antibody
Iba1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Iba1 -
由来種
Rabbit -
特異性
In WB, we recommend blocking in milk. Blocking with BSA gives high background. If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846 -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Iba1 (C terminal).
Database link: P55008 -
ポジティブ・コントロール
- Iba1 (Human) full length recombinant protein; Rat brain lysate; Human fetal lymphocytes; spleen tissue lysate; THP1, C6, NR8383 whole cell lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 17 kDa.
We recommend blocking in 3-5% milk. Blocking with BSA gives high background. |
|
IHC-P | (3) |
1/100 - 1/500.
|
特記事項 |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 17 kDa. We recommend blocking in 3-5% milk. Blocking with BSA gives high background. |
IHC-P
1/100 - 1/500. |
ターゲット情報
-
機能
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
組織特異性
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
配列類似性
Contains 2 EF-hand domains. -
翻訳後修飾
Phosphorylated on serine residues. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
-
参照データベース
- Entrez Gene: 199 Human
- Entrez Gene: 11629 Mouse
- Entrez Gene: 29427 Rat
- Omim: 601833 Human
- SwissProt: P55008 Human
- SwissProt: O70200 Mouse
- SwissProt: P55009 Rat
- Unigene: 76364 Human
see all -
別名
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
画像
-
IHC image of Iba1 staining in normal mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
IHC image of Iba1 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
IHC image of Iba1 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
ab108539, at 1/100 dilution, staining Iba1 in formalin-fixed, paraffin-embedded Human fetal lymphocytes by Immunohistochemistry.
-
All lanes : Anti-Iba1 antibody (ab108539) at 1/1000 dilution
Lane 1 : Human Iba1 recombinant protein at 0.1 µg
Lane 2 : HEK293 whole cell lysate at 20 µg
Lane 3 : A431 whole cell lysate at 20 µg
Lane 4 : NIH3T3 whole cell lysate at 30 µg
Lane 5 : Human spleen tissue lysate at 20 µg
Lane 6 : Mouse spleen tissue lysate at 30 µg
Lane 7 : Rat spleen tissue lysate at 30 µg
Lane 8 : U937 whole cell lysate at 30 µg
Lane 9 : MOLT4 whole cell lysate at 20 µg
Lane 10 : THP1 whole cell lysate at 30 µg
Lane 11 : THP1 whole cell lysate, PMA treated at 30 µg
Lane 12 : Raw 264.7 whole cell lysate at 30 µg
Lane 13 : C6 whole cell lysate at 30 µg
Lane 14 : NR8383 whole cell lysate at 30 µg
Lane 15 : NIH3T3 whole cell lysate - BLOCKED IN 5% BSA at 30 µg
Lane 16 : Human spleen tissue lysate - BLOCKED IN 5% BSA at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Exposure time: 3 minutesLanes 1-14: Blocked in 3% milk for 1 hour (RT). Lanes 15-16: Blocked in 5% BSA for 1 hour (RT).
Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab108539 (anti-Iba1 antibody; 1/1000) for 18 hours at 4°C. Antibody binding was detected using HRP-labelled anti-Rabbit IgG for 1 hour at room temperature and visualised using ECL development solution ab133406.
-
Anti-Iba1 antibody (ab108539) at 1/500 dilution + Rat brain lysate
Predicted band size: 17 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (39)
ab108539 は 39 報の論文で使用されています。
- Ammar RA et al. Neuroprotective effect of liraglutide in an experimental mouse model of multiple sclerosis: role of AMPK/SIRT1 signaling and NLRP3 inflammasome. Inflammopharmacology 30:919-934 (2022). PubMed: 35364735
- Jarabicová I et al. Analysis of necroptosis and its association with pyroptosis in organ damage in experimental pulmonary arterial hypertension. J Cell Mol Med 26:2633-2645 (2022). PubMed: 35393789
- Zammit NW et al. TNFAIP3 Reduction-of-Function Drives Female Infertility and CNS Inflammation. Front Immunol 13:811525 (2022). PubMed: 35464428
- Ibrahim WW & Abdel Rasheed NO Diapocynin neuroprotective effects in 3-nitropropionic acid Huntington's disease model in rats: emphasis on Sirt1/Nrf2 signaling pathway. Inflammopharmacology 30:1745-1758 (2022). PubMed: 35639233
- Petrovic A et al. Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica. Front Cell Neurosci 15:661492 (2021). PubMed: 33815068