Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (lung cancer cell lines A549, H23)
Specification
lung cancer cell lines A549, H23
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Vector Labs Rabbit anti-Mouse
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: ca. 180 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
lung cancer cell lines A549, H23
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 25 2014