Anti-IMPACT 抗体 (ab95175)
Key features and details
- Rabbit polyclonal to IMPACT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IMPACT antibody
IMPACT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IMPACT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 270-320 (GILLGPDRFK HINNCARNIL VEKNYTNSPE ESSKALGKNK KVRKDKKRNE H) of Human IMPACT (NP_060909.1).
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab95175 was affinity purified using an epitope specific to IMPACT immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95175の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 36 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. -
組織特異性
Widely expressed. Expressed at high level in brain. -
配列類似性
Belongs to the IMPACT family.
Contains 1 RWD domain. - Information by UniProt
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参照データベース
- Entrez Gene: 55364 Human
- SwissProt: Q9P2X3 Human
- Unigene: 515317 Human
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別名
- IMPACT antibody
- Impact homolog (mouse) antibody
- Impact homolog antibody
see all
画像
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All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds -
Detection of IMPACT in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab95175 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml
Lane 1 : IP with IMPACT antibody
Lane 2 : IP with ab95175
Lane 3 : IP with control IgG
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab95175 は論文での使用が確認できていません。