Anti-ILF3 抗体 (ab89100)
Key features and details
- Mouse polyclonal to ILF3
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ILF3 antibody
ILF3 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to ILF3 -
由来種
Mouse -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human ILF3 aa 1-750. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_004507 -
ポジティブ・コントロール
- WB: Human pancreas or HeLa nuclear lysate. IHC-P: Human small intestine tissue. ICC: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89100の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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特記事項 |
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ICC
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
ターゲット情報
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機能
May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
組織特異性
Ubiquitous. -
配列類似性
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 DZF domain. -
翻訳後修飾
Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
Methylated by protein arginine N-methyltransferase 1.
Arg-609 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 3609 Human
- Omim: 603182 Human
- SwissProt: Q12906 Human
- Unigene: 465885 Human
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別名
- CBTF antibody
- Double stranded RNA binding protein 76 antibody
- Double-stranded RNA-binding protein 76 antibody
see all
画像
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Anti-ILF3 antibody (ab89100) at 1 µg/ml + Human pancreas lysate at 50 µg
Predicted band size: 95 kDa
Observed band size: 80,95 kDa why is the actual band size different from the predicted? -
Anti-ILF3 antibody (ab89100) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 95 kDa
Observed band size: 95 kDa
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-ILF3 antibody (ab89100) at 1 µg/ml
Lane 1 : 293T transfected cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 95 kDa
Observed band size: 95 kDa -
ab89100, at 3µg/ml, staining human ILF3in small intestine, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
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Immunocytochemistry analysis of HeLa cells labeling ILF3 with ab89100 at 10 µg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab89100 は 2 報の論文で使用されています。
- Liu Y et al. Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop. J Exp Clin Cancer Res 38:439 (2019). PubMed: 31747963
- Zhuang Q et al. Long noncoding RNA lncAIS downregulation in mesenchymal stem cells is implicated in the pathogenesis of adolescent idiopathic scoliosis. Cell Death Differ 26:1700-1715 (2019). PubMed: 30464226