HRP Anti-ILF3 抗体 [EPR3626] (ab206250)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR3626] to ILF3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-ILF3 antibody [EPR3626]
ILF3 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [EPR3626] to ILF3 -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Raji, HeLa and K562 whole cell lysates. IHC-P: normal human kidney tissue sections
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3626 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab206250の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/5000. Detects a band of approximately 95,110 kDa (predicted molecular weight: 95 kDa).
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特記事項 |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 95,110 kDa (predicted molecular weight: 95 kDa). |
ターゲット情報
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機能
May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
組織特異性
Ubiquitous. -
配列類似性
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 DZF domain. -
翻訳後修飾
Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
Methylated by protein arginine N-methyltransferase 1.
Arg-609 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 3609 Human
- Entrez Gene: 16201 Mouse
- Omim: 603182 Human
- SwissProt: Q12906 Human
- SwissProt: Q9Z1X4 Mouse
- Unigene: 465885 Human
- Unigene: 440026 Mouse
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別名
- CBTF antibody
- Double stranded RNA binding protein 76 antibody
- Double-stranded RNA-binding protein 76 antibody
see all
画像
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All lanes : HRP Anti-ILF3 antibody [EPR3626] (ab206250) at 1/5000 dilution
Lane 1 : Raji Whole Cell lysate
Lane 2 : HeLa Whole Cell lysate
Lane 3 : K562 Whole Cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 95 kDaBlocking buffer: 3% Milk
Gel type: MOPS
Observed band size: 95 kDa
Exposure Time: 2 minutes
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IHC image of ILF3 staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab206250, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab206250 は 2 報の論文で使用されています。
- Pham T et al. Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide. Molecules 26:N/A (2021). PubMed: 33921211
- Liao R et al. Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity. Front Cell Dev Biol 8:614624 (2020). PubMed: 33585449