Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (stem cells)
Specification
stem cells
Fixative
Methanol
Permeabilization
No
Blocking step
Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 30 minute(s) · Temperature: 22°C · Diluent: 10% diluted milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rat IgG, Cy5-Conjugate (AP136S)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Dilution
1/100
Additional data
Additional Notes
Photograph from a laser scanning microscope.
Laser emmission wave length: 633 nm.
Pink is a false color.
Spheroids formed from mouse stem cells.
Laser emmission wave length: 633 nm.
Pink is a false color.
Spheroids formed from mouse stem cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
PROF. U.R. Markert
Verified customer
投稿 Feb 08 2013