Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Tris, 1mM EDTA pH 9
Sample
Monkey Tissue sections (retina)
Specification
retina
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 2.5 % serum/PBS
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: alexafluor
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/400
Additional data
Additional Notes
Macaque retina tissue fixed in 4% PFA/PBS. IF on paraffin embedded tissue. ab9324 stained retina tissue brightly. Ab9324 also stained parrafin embedded HeLa cells as positive control. Rhodamine(red)= IL-6 staining. DAPI(blue)=Hoechst staining of nuclei.
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投稿 Feb 10 2015