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Product name
Anti-IL6 antibody
IL6 抗体 (34件) 一覧
Description
Rabbit polyclonal to IL6
Specificity
The antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule (21-28 kDa). The antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
Tested applications
WB, ELISA, IHC-P, RIA, IP, IHC-Fr, Neutralising, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Immunogen
recombinant human IL-6 produced in E.coli.
Positive control
WB: lysate of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)(PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS)
General notes
IL-6 synonyms: plasmacytoma growth factor (PCT-GF),interferon-a-2 (IFN-a2), monocyte derived human B cellgrowth factor, B cell stimulating factor (BSF-2),hepatocyte stimulating factor (HSF), and interleukinhybridoma/plasmacytoma-1 (IL-HP1).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Purity
Whole antiserum
Purification notes
This antiserum has been heated to 56°C for 30 minutes.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Metabolic disorders
Metabolism >> Types of disease >> Cancer
Cancer >> Tumor immunology >> Cytokines >> Interleukins
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Neuroscience >> Neurology process >> Metabolism
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000.
ELISA
ELISA: 1/1000 - 1/5000.ELISA: 1/1000 - 1/5000.
IHC-P: 1/400 - 1/800.
RIA
RIA: 1/4000 - 1/8000.RIA: 1/4000 - 1/8000.
IP
IP: 1/400 - 1/800.IP: 1/400 - 1/800.
IHC-Fr: 1/400 - 1/800.
Neutralising
Neut: 1/400.Neut: 1/400.
ICC/IF: 1/500. See Abreview.
Target
Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
Sequence similarities
Belongs to the IL-6 superfamily.
Post-translational
modifications
N- and O-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP05231
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Database links
- Entrez Gene: 3569 Human
- Entrez Gene: 16193 Mouse
- Entrez Gene: 399500 Pig
- Entrez Gene: 24498 Rat
- Omim: 147620 Human
- SwissProt: P05231 Human
- SwissProt: P08505 Mouse
- SwissProt: P26893 Pig
see all
Anti-IL6 antibody images:
Western blot - IL6 antibody (ab6672)
Anti-IL6 antibody (ab6672) at 1/500 dilution + Endotoxin-stimulated human peripheral blood mononuclear cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IL6 antibody (ab6672)
The samples are formalin-fixed, formic acid decalcified rat knee joints with type II collagen induced arthritis. This image was kindly supplied as part of the review submitted by Elizabeth Chlipala (at University of Colorado, USA).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IL6 antibody (ab6672)
This picture represents a tissue section from the wrist joint in collagen-induced arthritis in mice stained with ab6672. This image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
Western blot - IL6 antibody (ab6672)
Anti-IL6 antibody (ab6672) at 1/1000 dilution
Figure. Immunoblot using Abcam's anti-
IL6 antibody. This blot shows detection of an
IL-6 26kDa tagged fusion protein (~300 ng, lane 1,
green, 800 nm channel). Minimal reactivity is
observed against the tag (data not shown).
Protein was resolved on a 4-20% Tris-
Glycine gel by SDS-PAGE and transferred
onto nitrocellulose. After blocking, the
membrane was probed with the primary
antibody diluted to 1:1,000. Incubation was
overnight at 4° C followed by washes and
reaction with a 1:10,000 dilution of a fluorescent conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room
temperature. Molecular weight markers are
shown for size comparison (red, 700
nm channel). Other detection systems will yield similar
results.
Immunocytochemistry/ Immunofluorescence - IL6 antibody (ab6672)
ab6672 staining IL6 in human WBC by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in PBS; 0.1% TX10; 1% Goat serum) for 16 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Frozen sections) - IL6 antibody (ab6672)
ab6672 staining IL6 in Mouse pancreatic neoplastic lesion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS + Triton (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-mrabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained with DAPI.
This image is courtesy of an anonymous Abreview
References for Anti-IL6 antibody (ab6672)
This product has been referenced in:
- Alcazar MA et al. Early postnatal hyperalimentation impairs renal function via SOCS-3 mediated renal postreceptor leptin resistance. Endocrinology 153:1397-410 (2012). Rat.Read more (PubMed: 22253420) »
- Lau K et al. Inhibition of type 1 diabetes correlated to a Lactobacillus johnsonii N6.2-mediated Th17 bias. J Immunol 186:3538-46 (2011).Read more (PubMed: 21317395) »
See all 24 publications for this product
Publishing research using ab6672? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"