Anti-IL-33 抗体 (ab118503)
Key features and details
- Rabbit polyclonal to IL-33
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IL-33 antibody
IL-33 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-33 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ELISAmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human IL-33 (internal sequence) conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- Human lymph node lysate and uterus tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118503の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). -
組織特異性
Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. -
配列類似性
Belongs to the IL-1 family. Highly divergent. -
ドメイン
The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association. -
翻訳後修飾
The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. -
細胞内局在
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). - Information by UniProt
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参照データベース
- Entrez Gene: 90865 Human
- Entrez Gene: 77125 Mouse
- Entrez Gene: 361749 Rat
- Omim: 608678 Human
- SwissProt: O95760 Human
- SwissProt: Q8BVZ5 Mouse
- SwissProt: Q66H70 Rat
- Unigene: 731660 Human
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別名
- C9orf26 antibody
- CHROMOSOME 9 OPEN READING FRAME 26 antibody
- DKFZp586H0523 antibody
see all
画像
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Immunohistochemical staining of paraffin-embedded, formalin fixed, human tonsil lymphocytes with ab118503 at 5 µg/mL.
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Negative control - immunohistochemical staining of paraffin-embedded, formalin fixed, human seminiferous tubules with ab118503 at 5 µg/mL.
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Human Uterus, Vessels (formalin-fixed, paraffin-embedded) stained with IL33 antibody ab118503 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Lane 1 : Anti-IL-33 antibody (ab118503) at 1 µg/ml
Lane 2 : Anti-IL-33 antibody (ab118503) at 2 µg/ml
All lanes : Human lymph node tissue lysate
Predicted band size: 31 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab118503 は 5 報の論文で使用されています。
- Chenery AL et al. IL-13 deficiency exacerbates lung damage and impairs epithelial-derived type 2 molecules during nematode infection. Life Sci Alliance 4:N/A (2021). PubMed: 34127548
- Sun Z et al. Plasma levels of soluble ST2, but not IL-33, correlate with the severity of alcoholic liver disease. J Cell Mol Med N/A:N/A (2018). PubMed: 30478965
- Chen M et al. Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction. Evid Based Complement Alternat Med 2017:2489842 (2017). PubMed: 28751921
- Sun L et al. Serum amyloid A induces interleukin-33 expression through an IRF7-dependent pathway. Eur J Immunol 44:2153-64 (2014). PubMed: 24777946
- Sun Y et al. Interleukin-33 promotes disease progression in patients with primary biliary cirrhosis. Tohoku J Exp Med 234:255-61 (2014). PubMed: 25400121