製品の概要
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製品名Anti-IL3 antibody
IL3 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to IL3
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由来種Rabbit
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アプリケーション適用あり: WB, ELISA, Neutralisingmore details
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種交差性交差種: Human
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免疫原
Highly pure (>98%) recombinant hIL-3 (human Interleukin-3)
製品の特性
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製品の状態Lyophilised:Reconstitute with 200µl of sterile water.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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バッファーPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプunknown
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軽鎖の種類unknown
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab9620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| ELISA | ||
| Neutralising |
追加情報
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3 (2.0 ng/ml), a concentration of 0.06 - 0.1 µg/ml of this antibody is required.
ELISA:To detect hIL-3 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-3.
Western Blot:To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA:To detect hIL-3 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-3.
Western Blot:To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ターゲット情報
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機能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
組織特異性Activated T-cells, mast cells, natural killer cells.
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配列類似性Belongs to the IL-3 family.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 3562 Human
- Omim: 147740 Human
- SwissProt: P08700 Human
- Unigene: 694 Human
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別名
- Colony stimulating factor multiple antibody
- Hematopoietic growth factor antibody
- IL 3 antibody
see all
プロトコール
参考文献
This product has been referenced in:
- Miron VE et al. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination. Nat Neurosci 16:1211-8 (2013). Read more (PubMed: 23872599) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"