Application
ELISA
Sample
Human Recombinant protein (Human recombinant IL-1RA from PeproTech)
Specification
Human recombinant IL-1RA from PeproTech
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Sandwich (Detection)
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1xPBS 0.05% Tween-20 1% BSA
Dilution
1/2500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Signal detection:
1:200 Streptavidin-HRP, incubation for 20 min at room temperature
wash
TMB, incubation for 15 min at room temperature
1M H2SO4 to stop reaction
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投稿 Mar 19 2014