Anti-IL-10 抗体 (ab34843)
Key features and details
- Rabbit polyclonal to IL-10
- Suitable for: RIA, Neutralising, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IL-10 antibody
IL-10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-10 -
由来種
Rabbit -
アプリケーション
適用あり: RIA, Neutralising, WB, ELISAmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Full length native protein (purified) corresponding to Human IL-10.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34843の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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RIA |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration.
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
ELISA |
Use at an assay dependent concentration.
(as detection antibody, using a monoclonal antibody for capture). |
特記事項 |
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RIA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
ELISA
Use at an assay dependent concentration. (as detection antibody, using a monoclonal antibody for capture). |
ターゲット情報
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機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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別名
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
画像
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Anti-IL-10 antibody (ab34843) at 1/200 dilution + recombinant IL-10 protein
Secondary
Goat anti-rabbit at 1/20000 dilution
Predicted band size: 21 kDa
4-20% reducing gel.Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.Dilution buffer: 3% BSA-TBS.Incubated O/N at 4°C. -
Anti-IL-10 antibody (ab34843) at 1/500 dilution + recombinant IL-10 protein, 50ng
Secondary
Peroxidase conjugated secondary at 1/40000 dilution
Predicted band size: 21 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (84)
ab34843 は 84 報の論文で使用されています。
- Tauber Z & Cizkova K The anti-inflammatory role of placental Hofbauer cells is altered in patients with chorioamnionitis: Are CYP2C8 and soluble epoxide hydrolase involved in immunomodulation? Biomed Pap Med Fac Univ Palacky Olomouc Czech Repu 166:267-273 (2022). PubMed: 33976432
- Niu X et al. Serum amyloid A 1 induces suppressive neutrophils through the Toll-like receptor 2-mediated signaling pathway to promote progression of breast cancer. Cancer Sci 113:1140-1153 (2022). PubMed: 35102665
- Ravi VM et al. T-cell dysfunction in the glioblastoma microenvironment is mediated by myeloid cells releasing interleukin-10. Nat Commun 13:925 (2022). PubMed: 35177622
- Li Y et al. Microbial and metabolic profiles of bronchopulmonary dysplasia and therapeutic effects of potential probiotics Limosilactobacillus reuteri and Bifidobacterium bifidum. J Appl Microbiol 133:908-921 (2022). PubMed: 35488863
- Teng YY et al. Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis. Front Bioeng Biotechnol 10:902894 (2022). PubMed: 35832407