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Anti-IL1 beta antibody (ab34837)

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Overview

Product name

Anti-IL1 beta antibody
IL1 beta 抗体 (28件) 一覧

Description

Rabbit polyclonal to IL1 beta

Specificity

This antibody primarily recognizes the 17kDa mature form of IL1 beta. It also recognizes 10% of the precursor, 31kDa form of IL1 beta in cell lysates. The antibody does not react with IL1 alpha.

Tested applications

ELISA, RIA, WB, IP, IHC-P, IHC-Fr, Neutralisingmore details

Cross reactivity

Reacts with

Dog, Human

Predicted to work with

Non Human Primates

Does not react with

Mouse, Rat, Rabbit

Immunogen

Recombinant IL1 beta (Human) produced in E.coli. The MW of the recombinant 153 amino acid IL1 beta was 17 kDa with the N terminal amino acid at position alanine 117. This cleavage site is generated by the IL1 beta converting enzyme (ICE, Capase 1).

Positive control

Supernatants or cell lysates from cultures of LPS stimulated human peripheral blood mononuclear cells. (PBMCs were stimulated for 24 hours with 1% human serum and 10ng/ml E.coli derived LPS.)

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: Whole serum

Purity

Whole antiserum

Purification notes

This antibody is sterile filtered and has been heated at 56°C for 30 minutes. Endotoxin content: <10pg/µl by LAL method.

一次抗体 notes

For Western blotting, heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1 beta precursor molecule, which often migrates as a 35,000 kDa band. This is due to the unfolding of the denatured precursor IL1 beta exposing epitopes not exposed in the natural state.

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Obesity
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Immunology >> Innate Immunity >> Cytokines >> Interleukins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab34837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/500 - 1/5000.

    ELISA: 1/500 - 1/5000.

  • RIA

     RIA: 1/5000.

    RIA: 1/5000.

  • ShowHide

    WB

      See more...Read more →

    WB: Use 2µg for 106 cells. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

  • IP

     IP: 1/400 - 1/800.

    IP: 1/400 - 1/800.

  • IHC-P

     IHC-P: 1/200 - 1/400.

    IHC-P: 1/200 - 1/400.

  • IHC-Fr

     IHC-Fr: 1/200 - 1/400.

    IHC-Fr: 1/200 - 1/400.

  • ShowHide

    Neutralising

     Neut: 1/200. Incubate wi...Read more →

    Neut: 1/200. Incubate with antiserum for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG is recommended.

Target

Function

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Sequence similarities

Belongs to the IL-1 family.

Domain

The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.

Cellular localization

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Target information above from: UniProt accessionP01584 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Catabolin antibody
  • H1 antibody
  • Hematopoietin 1 antibody
  • IFN beta inducing factor antibody
  • IL 1 antibody
  • IL 1 beta antibody
  • IL 1B antibody
  • IL-1 beta antibody
  • IL1 BETA antibody
  • IL1B antibody
  • IL1B_HUMAN antibody
  • IL1F2 antibody
  • Interleukin 1 beta antibody
  • Interleukin 1 beta precursor antibody
  • Interleukin-1 beta antibody
  • LAF antibody
  • OAF antibody
  • Osteoclast activating factor antibody
  • OTTHUMP00000162031 antibody
  • Preinterleukin 1 beta antibody
  • Preinterleukin beta antibody
  • Pro interleukin 1 beta antibody
see all

Database links

References for Anti-IL1 beta antibody (ab34837)

ab34837 has not yet been referenced specifically in any publications.

Publishing research using ab34837? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"