Application
Western blot
Sample
Human Cell lysate - whole cell (peripheral blood mononuclear cells)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Loading amount
25 µg
Treatment
see below
Specification
peripheral blood mononuclear cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminescence
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ~35 kDa
Positive control
rh IL-1beta protein
Additional data
Additional Notes
lanes:
1= PBMC`s unstimulated
2 = Monocytes unstimualted
3 = Monocytes stimulated with LPS
4 = positive control (rh IL-1beta protein)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 07 2013