Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human primary tonsillar epithelial cell)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Human primary tonsillar epithelial cell
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: HINGS (blocking agent)
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa-Fluor-594 Goat anti-rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
These primary epithelial cells are from a human patient who has undergone a tonsillectomy. Cells are routinely cultured in specialist serum-free keratinocyte medium supplemented with bovine pituitary extract.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mhairi Morris
Verified customer
投稿 Sep 09 2009