Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 chicken anti-rabbit antibody
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
The antibody gave weak signal and relatively strong background, it does show cytoplasmic staining but not as strong as I wanted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 24 2007