Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Raw 264.7)
Total protein in input
100 µg
Specification
Raw 264.7
Treatment
2 hrs LPS
Immuno-precipitation step
Protein G
Other product details
Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
This antibody was used to precipitate IKKa as a positive control, it precipitated the correct size band but not with excellent efficiency hence the 3 stars for this application.
Figure: 10% of pre-IP material was loaded in lane 1. Pre-IP material was then split for 8 IPs (2 of which are shown IgG and IKKa), precipitated material was washed, eluted and all of it was loaded onto a 10% gel.
Figure: 10% of pre-IP material was loaded in lane 1. Pre-IP material was then split for 8 IPs (2 of which are shown IgG and IKKa), precipitated material was washed, eluted and all of it was loaded onto a 10% gel.
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投稿 Jul 31 2012