Anti-IKAP/p150 抗体 (ab62498)
Key features and details
- Rabbit polyclonal to IKAP/p150
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-IKAP/p150 antibody
IKAP/p150 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IKAP/p150 -
由来種
Rabbit -
特異性
This antibody reacts with IKAP/p150
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アプリケーション
適用あり: WB, ICC, ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human IKAP/p150.
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ポジティブ・コントロール
- A20 cell lysate and A20 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62498の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa).
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ICC |
Use a concentration of 1 - 20 µg/ml.
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ICC/IF |
Use a concentration of 1 - 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa). |
ICC
Use a concentration of 1 - 20 µg/ml. |
ICC/IF
Use a concentration of 1 - 20 µg/ml. |
ターゲット情報
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機能
May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. -
関連疾患
Defects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births. -
配列類似性
Belongs to the ELP1/IKA1 family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 230233 Mouse
- SwissProt: Q7TT37 Mouse
- Unigene: 282743 Mouse
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別名
- DKFZp781H1425 antibody
- DYS antibody
- Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
see all
画像
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Lane 1 : Anti-IKAP/p150 antibody (ab62498) at 0.5 µg/ml
Lane 2 : Anti-IKAP/p150 antibody (ab62498) at 1 µg/ml
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG
Predicted band size: 148 kDa
Observed band size: 100,148 kDa why is the actual band size different from the predicted? -
Immunocytochemistry of IKAP/p150 in A20 cells with ab62498 antibody at 1 µg/ml.
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Immunofluorescence of IKAP/p150 in A20 cells using ab62498 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab62498 は 1 報の論文で使用されています。
- Chen WT et al. Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation. J Biomed Sci 28:81 (2021). PubMed: 34819065