Anti-IGJ 抗体 [SP105] (ab105229)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP105] to IGJ
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IGJ antibody [SP105]
IGJ 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP105] to IGJ -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human IGJ aa 50-150 (internal sequence). The exact sequence is proprietary.
Database link: P01591 -
ポジティブ・コントロール
- IHC-P: Human tonsil and stomach tissue Flow Cyt (Intra): Daudi cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP105 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105229の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
|
Flow Cyt (Intra) |
1/20.
|
特記事項 |
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IHC-P
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Flow Cyt (Intra)
1/20. |
ターゲット情報
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機能
Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3512 Human
- Entrez Gene: 16069 Mouse
- Omim: 147790 Human
- SwissProt: P01591 Human
- SwissProt: P01592 Mouse
- Unigene: 643431 Human
- Unigene: 1192 Mouse
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別名
- IGCJ antibody
- IGJ_HUMAN antibody
- Immunoglobulin J chain antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells of human stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature. -
Flow cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) labeling IGJ with purified ab105229 at 1/20 dilution (5.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IGJ with ab105229 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105229 for 10 mins at room temperature. -
ab105229, at 1/100 dilution, staining IGJ in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab105229 は論文での使用が確認できていません。