Anti-IGF2BP2/IMP-2 抗体 [EPR6741(B)] (ab124930)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6741(B)] to IGF2BP2/IMP-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)]
IGF2BP2/IMP-2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6741(B)] to IGF2BP2/IMP-2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human IGF2BP2/IMP-2.
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ポジティブ・コントロール
- 293T, Caco2, HepG2, HeLa and SH SY5Y cell lysates, rat brain and mouse heart lysate; Human colon and human lung carcinoma tissue.
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特記事項
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.00 x 10 -12 M Learn more about KD -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6741(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/5000. Detects a band of approximately 55-65 kDa (predicted molecular weight: 66 kDa).
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IHC-P | (1) |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/2000 - 1/5000. Detects a band of approximately 55-65 kDa (predicted molecular weight: 66 kDa). |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. -
組織特異性
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. -
配列類似性
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
ドメイン
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
細胞内局在
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. - Information by UniProt
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参照データベース
- Entrez Gene: 10644 Human
- Entrez Gene: 319765 Mouse
- Entrez Gene: 303824 Rat
- Omim: 608289 Human
- SwissProt: Q9Y6M1 Human
- SwissProt: Q5SF07 Mouse
- Unigene: 35354 Human
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別名
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
see all
画像
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All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/5000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 60,62 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBSTThe double bands are consistent with what has been described in PMID 23177649.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling IGF2BP2/IMP-2 with purified ab124930 at 1/200 dilution (4.8 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling IGF2BP2/IMP-2 with purified ab124930 at 1/250 dilution (3.8μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/10000 dilution (purified) + Mouse heart lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/2000 dilution (purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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ab124930 (unpurified), at 1/50 dilution, staining IGF2BP2/IMP-2 in formalin-fixed, paraffin-embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab124930 は 13 報の論文で使用されています。
- Lin SH et al. Cytoplasmic IGF2BP2 Protein Expression in Human Patients with Oral Squamous Cell Carcinoma: Prognostic and Clinical Implications. Int J Med Sci 19:1198-1204 (2022). PubMed: 35919812
- Sa R et al. AhR Antagonist Promotes Differentiation of Papillary Thyroid Cancer via Regulating circSH2B3/miR-4640-5P/IGF2BP2 Axis. Front Pharmacol 12:795386 (2021). PubMed: 35002727
- Han L et al. IGF2BP2 Regulates MALAT1 by Serving as an N6-Methyladenosine Reader to Promote NSCLC Proliferation. Front Mol Biosci 8:780089 (2021). PubMed: 35111811
- Sekulovski N et al. Niclosamide's potential direct targets in ovarian cancer†. Biol Reprod 105:403-412 (2021). PubMed: 33855343
- Ma YS et al. microRNA-320b suppresses HNF4G and IGF2BP2 expression to inhibit angiogenesis and tumor growth of lung cancer. Carcinogenesis 42:762-771 (2021). PubMed: 33758932