Application
Western blot
Sample
Bos taurus Cell lysate - other (HEK 293T and BoMAC (SV40-transformed bovine macrop)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
HEK 293T and BoMAC (SV40-transformed bovine macrop
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Positive control
HEK 293T - Specific band in lysate supernatant: approximately 70 kDa and
unspecific band in lysate pellet: approximately 200 kDa
Additional data
Additional Notes
1st lane = lysate pellet HEK293T cells (human)
2nd lane = lysate supernatant HEK293T cells (human)
3rd lane = lysate pellet bovine macrophages
4th lane= lysate supernatant bovine macrophages
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback about the lack of cross-reactivity with bovine IGF2BP1. The homology of the immunogen and bovine IGF2BP1 is 100%, so we would have expected a cross-reactivity, however, based on the reviewers data, the antibody may not be suitable for cow. We welcome any further feedback and AbReviews from other scientists who have used this antibody in cow.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 19 2017